Seroreactivity clarification and viral load quantitation in HIV-1 and HIV-2 infections in Ghana.
Identifieur interne : 001176 ( Main/Exploration ); précédent : 001175; suivant : 001177Seroreactivity clarification and viral load quantitation in HIV-1 and HIV-2 infections in Ghana.
Auteurs : W K Ampofo [Japon] ; Y. Koyanagi ; J. Brandful ; K. Ishikawa ; N. YamamotoSource :
- Journal of medical and dental sciences [ 1342-8810 ] ; 1999.
Descripteurs français
- KwdFr :
- ARN viral (analyse), Anticorps anti-VIH (biosynthèse), Anticorps anti-VIH (sang), Charge virale, Ghana (épidémiologie), Humains, Infections à VIH (sang), Infections à VIH (virologie), Infections à VIH (épidémiologie), Numération des lymphocytes, Prévalence, RT-PCR, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (isolement et purification), VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (isolement et purification), Études séroépidémiologiques, Études transversales.
- MESH :
- analyse : ARN viral.
- biosynthèse : Anticorps anti-VIH.
- isolement et purification : VIH-1 (Virus de l'Immunodéficience Humaine de type 1), VIH-2 (Virus de l'Immunodéficience Humaine de type 2).
- sang : Anticorps anti-VIH, Infections à VIH.
- virologie : Infections à VIH.
- épidémiologie : Ghana, Infections à VIH.
- Charge virale, Humains, Numération des lymphocytes, Prévalence, RT-PCR, Études séroépidémiologiques, Études transversales.
- Wicri :
- geographic : Ghana.
English descriptors
- KwdEn :
- Cross-Sectional Studies, Ghana (epidemiology), HIV Antibodies (biosynthesis), HIV Antibodies (blood), HIV Infections (blood), HIV Infections (epidemiology), HIV Infections (virology), HIV-1 (isolation & purification), HIV-2 (isolation & purification), Humans, Lymphocyte Count, Prevalence, RNA, Viral (analysis), Reverse Transcriptase Polymerase Chain Reaction, Seroepidemiologic Studies, Viral Load.
- MESH :
- chemical , analysis : RNA, Viral.
- chemical , biosynthesis : HIV Antibodies.
- chemical , blood : HIV Antibodies.
- geographic , epidemiology : Ghana.
- blood : HIV Infections.
- epidemiology : HIV Infections.
- isolation & purification : HIV-1, HIV-2.
- virology : HIV Infections.
- Cross-Sectional Studies, Humans, Lymphocyte Count, Prevalence, Reverse Transcriptase Polymerase Chain Reaction, Seroepidemiologic Studies, Viral Load.
Abstract
In Ghana, West Africa, the prevalence of dual HIV-1 and HIV-2 infections remains to be clarified, and HIV viral load measurement is yet to be established. Conventional assays for HIV-1 RNA measurements have been limited specifically to HIV-1 subtype B, preventing their utilization for Ghana where HIV-1 subtypes A, D and G are prevalent. Therefore, we set out to distinguish the types of HIV infection existing in Ghana so as to determine the extent of actual dual infections, and to measure plasma HIV-1 RNA. Blood samples were collected from 563 sick and healthy Ghanaians who visited hospitals in 1996 and 1997. After T cells were counted, HIV antibody was screened and confirmed by six different commercial assays and one in-house test. Nested PCR was then used to verify HIV-1 and HIV-2 presence by type-specific primers. Plasma HIV-1 RNA was measured by an improved commercial RT-PCR assay, sensitive to all HIV-1 group M subtypes. HIV-1 alone (89%) clearly dominated over HIV-2 alone (2%), and HIV-1 and HIV-2 dual infections were found in 9%. Valid viral load measurements were obtained on test plasma representing the main HIV-1 subtype (A) prevailing in Ghana. A high amount of HIV-1 RNA (5.9 mean log10 RNA copies/ml) was observed in the typical stages of HIV infection represented by groups of CD44 cell counts. We have clarified the seroprevalence of HIV-1 and HIV-2 amongst HIV seropositives, and the high viral load of HIV-1 reflects its influence on AIDS in Ghana.
PubMed: 12160214
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000339
- to stream PubMed, to step Curation: 000339
- to stream PubMed, to step Checkpoint: 000339
- to stream Ncbi, to step Merge: 000062
- to stream Ncbi, to step Curation: 000062
- to stream Ncbi, to step Checkpoint: 000062
- to stream Main, to step Merge: 001263
- to stream Main, to step Curation: 001176
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Seroreactivity clarification and viral load quantitation in HIV-1 and HIV-2 infections in Ghana.</title><author><name sortKey="Ampofo, W K" sort="Ampofo, W K" uniqKey="Ampofo W" first="W K" last="Ampofo">W K Ampofo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Faculty of Medicine, Tokyo Medical and Dental University</wicri:regionArea><wicri:noRegion>Tokyo Medical and Dental University</wicri:noRegion></affiliation></author><author><name sortKey="Koyanagi, Y" sort="Koyanagi, Y" uniqKey="Koyanagi Y" first="Y" last="Koyanagi">Y. Koyanagi</name></author><author><name sortKey="Brandful, J" sort="Brandful, J" uniqKey="Brandful J" first="J" last="Brandful">J. Brandful</name></author><author><name sortKey="Ishikawa, K" sort="Ishikawa, K" uniqKey="Ishikawa K" first="K" last="Ishikawa">K. Ishikawa</name></author><author><name sortKey="Yamamoto, N" sort="Yamamoto, N" uniqKey="Yamamoto N" first="N" last="Yamamoto">N. Yamamoto</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1999">1999</date><idno type="RBID">pubmed:12160214</idno><idno type="pmid">12160214</idno><idno type="wicri:Area/PubMed/Corpus">000339</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000339</idno><idno type="wicri:Area/PubMed/Curation">000339</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000339</idno><idno type="wicri:Area/PubMed/Checkpoint">000339</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000339</idno><idno type="wicri:Area/Ncbi/Merge">000062</idno><idno type="wicri:Area/Ncbi/Curation">000062</idno><idno type="wicri:Area/Ncbi/Checkpoint">000062</idno><idno type="wicri:doubleKey">1342-8810:1999:Ampofo W:seroreactivity:clarification:and</idno><idno type="wicri:Area/Main/Merge">001263</idno><idno type="wicri:Area/Main/Curation">001176</idno><idno type="wicri:Area/Main/Exploration">001176</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Seroreactivity clarification and viral load quantitation in HIV-1 and HIV-2 infections in Ghana.</title><author><name sortKey="Ampofo, W K" sort="Ampofo, W K" uniqKey="Ampofo W" first="W K" last="Ampofo">W K Ampofo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Faculty of Medicine, Tokyo Medical and Dental University</wicri:regionArea><wicri:noRegion>Tokyo Medical and Dental University</wicri:noRegion></affiliation></author><author><name sortKey="Koyanagi, Y" sort="Koyanagi, Y" uniqKey="Koyanagi Y" first="Y" last="Koyanagi">Y. Koyanagi</name></author><author><name sortKey="Brandful, J" sort="Brandful, J" uniqKey="Brandful J" first="J" last="Brandful">J. Brandful</name></author><author><name sortKey="Ishikawa, K" sort="Ishikawa, K" uniqKey="Ishikawa K" first="K" last="Ishikawa">K. Ishikawa</name></author><author><name sortKey="Yamamoto, N" sort="Yamamoto, N" uniqKey="Yamamoto N" first="N" last="Yamamoto">N. Yamamoto</name></author></analytic><series><title level="j">Journal of medical and dental sciences</title><idno type="ISSN">1342-8810</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cross-Sectional Studies</term><term>Ghana (epidemiology)</term><term>HIV Antibodies (biosynthesis)</term><term>HIV Antibodies (blood)</term><term>HIV Infections (blood)</term><term>HIV Infections (epidemiology)</term><term>HIV Infections (virology)</term><term>HIV-1 (isolation & purification)</term><term>HIV-2 (isolation & purification)</term><term>Humans</term><term>Lymphocyte Count</term><term>Prevalence</term><term>RNA, Viral (analysis)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Seroepidemiologic Studies</term><term>Viral Load</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>Anticorps anti-VIH (biosynthèse)</term><term>Anticorps anti-VIH (sang)</term><term>Charge virale</term><term>Ghana (épidémiologie)</term><term>Humains</term><term>Infections à VIH (sang)</term><term>Infections à VIH (virologie)</term><term>Infections à VIH (épidémiologie)</term><term>Numération des lymphocytes</term><term>Prévalence</term><term>RT-PCR</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (isolement et purification)</term><term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (isolement et purification)</term><term>Études séroépidémiologiques</term><term>Études transversales</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>HIV Antibodies</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>HIV Antibodies</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Ghana</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps anti-VIH</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>HIV Infections</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>HIV Infections</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>HIV-1</term><term>HIV-2</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term><term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2)</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps anti-VIH</term><term>Infections à VIH</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à VIH</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>HIV Infections</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Ghana</term><term>Infections à VIH</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cross-Sectional Studies</term><term>Humans</term><term>Lymphocyte Count</term><term>Prevalence</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Seroepidemiologic Studies</term><term>Viral Load</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Charge virale</term><term>Humains</term><term>Numération des lymphocytes</term><term>Prévalence</term><term>RT-PCR</term><term>Études séroépidémiologiques</term><term>Études transversales</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Ghana</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In Ghana, West Africa, the prevalence of dual HIV-1 and HIV-2 infections remains to be clarified, and HIV viral load measurement is yet to be established. Conventional assays for HIV-1 RNA measurements have been limited specifically to HIV-1 subtype B, preventing their utilization for Ghana where HIV-1 subtypes A, D and G are prevalent. Therefore, we set out to distinguish the types of HIV infection existing in Ghana so as to determine the extent of actual dual infections, and to measure plasma HIV-1 RNA. Blood samples were collected from 563 sick and healthy Ghanaians who visited hospitals in 1996 and 1997. After T cells were counted, HIV antibody was screened and confirmed by six different commercial assays and one in-house test. Nested PCR was then used to verify HIV-1 and HIV-2 presence by type-specific primers. Plasma HIV-1 RNA was measured by an improved commercial RT-PCR assay, sensitive to all HIV-1 group M subtypes. HIV-1 alone (89%) clearly dominated over HIV-2 alone (2%), and HIV-1 and HIV-2 dual infections were found in 9%. Valid viral load measurements were obtained on test plasma representing the main HIV-1 subtype (A) prevailing in Ghana. A high amount of HIV-1 RNA (5.9 mean log10 RNA copies/ml) was observed in the typical stages of HIV infection represented by groups of CD44 cell counts. We have clarified the seroprevalence of HIV-1 and HIV-2 amongst HIV seropositives, and the high viral load of HIV-1 reflects its influence on AIDS in Ghana.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Brandful, J" sort="Brandful, J" uniqKey="Brandful J" first="J" last="Brandful">J. Brandful</name><name sortKey="Ishikawa, K" sort="Ishikawa, K" uniqKey="Ishikawa K" first="K" last="Ishikawa">K. Ishikawa</name><name sortKey="Koyanagi, Y" sort="Koyanagi, Y" uniqKey="Koyanagi Y" first="Y" last="Koyanagi">Y. Koyanagi</name><name sortKey="Yamamoto, N" sort="Yamamoto, N" uniqKey="Yamamoto N" first="N" last="Yamamoto">N. Yamamoto</name></noCountry><country name="Japon"><noRegion><name sortKey="Ampofo, W K" sort="Ampofo, W K" uniqKey="Ampofo W" first="W K" last="Ampofo">W K Ampofo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/SidaGhanaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001176 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001176 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= SidaGhanaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:12160214
|texte= Seroreactivity clarification and viral load quantitation in HIV-1 and HIV-2 infections in Ghana.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:12160214" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SidaGhanaV1
| This area was generated with Dilib version V0.6.31. |  |